The 2nd sampling in the gwTriade project took place on November 20 and 21, 2023. The aim of this sampling was to investigate the quality of urban groundwater and the influence of the city on the quality and condition of the groundwater habitat. We sampled groundwater from nine different locations in the city of Hanover. Subsequently, we analyze the samples taken according to the triad approach for the groundwater organisms living in it, the anthropogenic chemicals present and the ecotoxicological effects of the samples on cells and organisms.